synapsin ii Search Results


synapsin ii  (Alomone Labs)
90
Alomone Labs synapsin ii
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Synapsin Ii, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synapsin 2  (Novus Biologicals)
90
Novus Biologicals synapsin 2
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Synapsin 2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti synapsin 2 antibody  (Novus Biologicals)
86
Novus Biologicals mouse anti synapsin 2 antibody
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Mouse Anti Synapsin 2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit syn2  (Proteintech)
92
Proteintech rabbit syn2
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Rabbit Syn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti αsma  (Biorbyt)
93
Biorbyt anti αsma
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Anti αsma, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit antibody against the a-domain of synapsin i/ii #106002  (Synaptic Systems)
90
Synaptic Systems polyclonal rabbit antibody against the a-domain of synapsin i/ii #106002
A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive <t>for</t> <t>AC3</t> (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses <t>(Synapsin</t> II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).
Polyclonal Rabbit Antibody Against The A Domain Of Synapsin I/Ii #106002, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s549 or s603 synaptic vesicle protein, synapsin i/ii  (Becton Dickinson)
90
Becton Dickinson s549 or s603 synaptic vesicle protein, synapsin i/ii
Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.
S549 Or S603 Synaptic Vesicle Protein, Synapsin I/Ii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-synapsin i/ii 106 002  (Synaptic Systems)
90
Synaptic Systems anti-synapsin i/ii 106 002
Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.
Anti Synapsin I/Ii 106 002, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibodies to synapsin ii  (QED Bioscience)
90
QED Bioscience rabbit polyclonal antibodies to synapsin ii
Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.
Rabbit Polyclonal Antibodies To Synapsin Ii, supplied by QED Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synapsin ii  (CH Instruments)
90
CH Instruments synapsin ii
Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.
Synapsin Ii, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human synapsin-ii gene promoter  (Brinkmann Instruments)
90
Brinkmann Instruments human synapsin-ii gene promoter
Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.
Human Synapsin Ii Gene Promoter, supplied by Brinkmann Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synapsin 2 (rabbit polyclonal “poly  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies synapsin 2 (rabbit polyclonal “poly
Fine structure and molecular phenotype of axon terminals in Reelin-OE mice. (A–C) Electron micrographs illustrating axon terminals establishing synaptic contacts (arrows) in the inner molecular layer of control, Reelin-OE, and DOX-treated Reelin-OE mice. (D–F) Histograms showing area (D) , circularity index (E) , and length of synaptic contact (F) of axon terminals in different hippocampal layers in control, Reelin-OE, and DOX-treated Reelin-OE mice (mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ANOVA test). (G) Western-blot analysis of a and b isoforms of Synapsin 2, and SNAP25 in synaptosomal fractions of the hippocampus. (H) Quantification of Synapsin 2a, Synapsin 2b, and SNAP-25 values from triplicate experiments. Values are normalized to the levels of actin and expressed as percentage of control mice (mean ± SEM, ANOVA test). IML, inner molecular layer; MML, medial molecular layer, OML, outer molecular layer; SLM, stratum lacunosum-moleculare; SO, stratum oriens; SR, stratum radiatum. Scale bar in (A–C) , 0.5 μm.
Synapsin 2 (Rabbit Polyclonal “Poly, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive for AC3 (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses (Synapsin II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).

Journal: bioRxiv

Article Title: GABA-induced Ca 2+ signaling in the primary cilium of neurons

doi: 10.1101/2025.05.26.656109

Figure Lengend Snippet: A. Immunofluorescence images from naïve or 5HT6-GGECO1-expressing cultured mouse neurons (from cortex and CA1) showing the distribution of GABA-B1 receptors (magenta) in primary cilia positive for AC3 (green). Intensity profiles from lines drawn along the cilia are shown to the right and further show the confinement of GABA-B1 receptors to the cilia base of cultured neurons. B. Quantification of GABA-B1 receptor enrichment at the cilia base in naïve cultured neurons or cultured cortical and hippocampal neurons expressing 5HT6-GGECO1 (naïve 2,76±0,37; Cx 8,18±0,91; Hipp 4,94±0,44 arbitrary units). GABA-B1 positive segment length was measured for each group and found significantly different (naïve, Cx and Hipp, in µm: 2,78±0,37; 8,18±0,91; 5,03±0,39; mean±SEM; Kruskal-Wallis ANOVA followed by multiple comparisons, naïve n=12, Cx n=22, Hipp n=38, for A & B). C. Simultaneous recording of cytoplasmic (grey) and ciliary (pink) calcium activities from a neuron before and after stimulation with 100nM baclofen. Activation of metabotropic GABA receptors was without effect on the frequency of somatic events while ciliary signaling was increased by the agonist. Positive deviations from baseline were computed over time to produce ciliary activity count traces (black, lower panel). D. Averaged activity counts corresponding to neurons in control (blue) baclofen (100nM, black) and TTX (1 µM) + baclofen (100nM, grey) showing an increase of activity after stimulation with baclofen. E. Quantification of activity changes was performed in a pair wise fashion, comparing the averaged activity during initial vs final period (control) and baseline vs baclofen (either in the absence of presence of TTX). Time lacked an effect on activity (control P=0,4332) while baclofen stimulation led to a significant increase in both - /+ TTX conditions (respectively P=0,0002 and P=0,0039; two tailed Wilcoxon matched-pairs signed rank test) F. To test whether abolition of action potentials had an impact on spontaneous ciliary activity, recordings in control and TTX (initial 20 minutes both groups) were compared. Statistical analysis showed no difference (P=0,1468 Mann-Whitney two tailed t-test). G. Immunofluorescence images showing the distribution of primary cilia (AC3; green) and synapses (Synapsin II; magenta) in cultured cortical neurons. Boxed areas are magnified to the right. Scatter plot to the left shows Synapsin II fluorescence intensity at primary cilia and random locations within the same sample (P=0,3696 Wilcoxon matched pairs signed rank test).

Article Snippet: The following primary antibodies (1/300 dilutions) were used: ARL13b (Abcam ab136648), AC3 (Abcam ab277619), AC3 (Alomone AAR-043), NeuN (Sigma-Aldrich ABN90), GFAP (Synaptic System 173–004), Synapsin II (Alomone ANR-015), GABA-B1 (Alomone AGB-001), MCHR1 (Thermo Fischer PA5-77492), Patched (Abcam ab53715), EP4 (Santa Cruz Biotechnology sc-55596), ANKS6 (Sigma HPA008355), NPHP3 (Proteintech 22026-1-AP), NEK8 (kind gift from Prof. David R. Beier, Seattle Children’s Hospital, USA).

Techniques: Immunofluorescence, Expressing, Cell Culture, Activation Assay, Activity Assay, Control, Two Tailed Test, MANN-WHITNEY, Fluorescence

Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.

Journal:

Article Title: Nerve Agent Exposure Elicits Site-Specific Changes in Protein Phosphorylation in Mouse Brain

doi: 10.1016/j.brainres.2010.04.034

Figure Lengend Snippet: Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.

Article Snippet: Immunoblotting was carried out using phosphorylation state-specific antibodies (and respective phosphorylation state insensitive antibodies for measuring the total protein levels) raised against pT34, pT75, pS102 or pS137 of DARPP-32 and pS94 of spinophilin (ITI); pS133 of CREB (Upstate Biotechnology, Charlottesville, VA); pT183 of ERK1/2 (Promega, Madison, WI); pS897 of the NMDA receptor NR1 subunit (Upstate); pS831 or pS845 of the AMPA receptor GluR1 subunit (Upstate); S9 of the GSK3β kinase (BD Biosciences); S473 or T308 of the protein kinase Akt (BD Biosciences); S549 or S603 of the synaptic vesicle protein, synapsin I/II (Dr. Angus Nairn, RU and BD Biosciences); or pS40 of tyrosine hydroxylase (Chemicon, Temecula, CA).

Techniques:

Sites of phosphorylation monitored in brain after DFP treatment using CNSProfile.

Journal:

Article Title: Nerve Agent Exposure Elicits Site-Specific Changes in Protein Phosphorylation in Mouse Brain

doi: 10.1016/j.brainres.2010.04.034

Figure Lengend Snippet: Sites of phosphorylation monitored in brain after DFP treatment using CNSProfile.

Article Snippet: Immunoblotting was carried out using phosphorylation state-specific antibodies (and respective phosphorylation state insensitive antibodies for measuring the total protein levels) raised against pT34, pT75, pS102 or pS137 of DARPP-32 and pS94 of spinophilin (ITI); pS133 of CREB (Upstate Biotechnology, Charlottesville, VA); pT183 of ERK1/2 (Promega, Madison, WI); pS897 of the NMDA receptor NR1 subunit (Upstate); pS831 or pS845 of the AMPA receptor GluR1 subunit (Upstate); S9 of the GSK3β kinase (BD Biosciences); S473 or T308 of the protein kinase Akt (BD Biosciences); S549 or S603 of the synaptic vesicle protein, synapsin I/II (Dr. Angus Nairn, RU and BD Biosciences); or pS40 of tyrosine hydroxylase (Chemicon, Temecula, CA).

Techniques: Activity Assay

Fine structure and molecular phenotype of axon terminals in Reelin-OE mice. (A–C) Electron micrographs illustrating axon terminals establishing synaptic contacts (arrows) in the inner molecular layer of control, Reelin-OE, and DOX-treated Reelin-OE mice. (D–F) Histograms showing area (D) , circularity index (E) , and length of synaptic contact (F) of axon terminals in different hippocampal layers in control, Reelin-OE, and DOX-treated Reelin-OE mice (mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ANOVA test). (G) Western-blot analysis of a and b isoforms of Synapsin 2, and SNAP25 in synaptosomal fractions of the hippocampus. (H) Quantification of Synapsin 2a, Synapsin 2b, and SNAP-25 values from triplicate experiments. Values are normalized to the levels of actin and expressed as percentage of control mice (mean ± SEM, ANOVA test). IML, inner molecular layer; MML, medial molecular layer, OML, outer molecular layer; SLM, stratum lacunosum-moleculare; SO, stratum oriens; SR, stratum radiatum. Scale bar in (A–C) , 0.5 μm.

Journal: Frontiers in Cellular Neuroscience

Article Title: Reelin Exerts Structural, Biochemical and Transcriptional Regulation Over Presynaptic and Postsynaptic Elements in the Adult Hippocampus

doi: 10.3389/fncel.2016.00138

Figure Lengend Snippet: Fine structure and molecular phenotype of axon terminals in Reelin-OE mice. (A–C) Electron micrographs illustrating axon terminals establishing synaptic contacts (arrows) in the inner molecular layer of control, Reelin-OE, and DOX-treated Reelin-OE mice. (D–F) Histograms showing area (D) , circularity index (E) , and length of synaptic contact (F) of axon terminals in different hippocampal layers in control, Reelin-OE, and DOX-treated Reelin-OE mice (mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ANOVA test). (G) Western-blot analysis of a and b isoforms of Synapsin 2, and SNAP25 in synaptosomal fractions of the hippocampus. (H) Quantification of Synapsin 2a, Synapsin 2b, and SNAP-25 values from triplicate experiments. Values are normalized to the levels of actin and expressed as percentage of control mice (mean ± SEM, ANOVA test). IML, inner molecular layer; MML, medial molecular layer, OML, outer molecular layer; SLM, stratum lacunosum-moleculare; SO, stratum oriens; SR, stratum radiatum. Scale bar in (A–C) , 0.5 μm.

Article Snippet: Western blots against various proteins of interest were performed using the following antibodies: Actin (mouse monoclonal “mAb” clone C4; 1:100000; Millipore), Synapsin 2 (rabbit polyclonal “poly”; 1:500; Stressgen Bioreagents), SNAP25 (mouse mAb clone SMI-81; 1:750; Becton-Dickinson), Synaptopodin (rabbit poly; 1:750; Synaptic Systems), NR2a (rabbit poly; 1:500; Millipore), NR2b (rabbit poly; 1:500; Millipore), p-Cofilin on serine 3 (rabbit poly; 1/100; Santa Cruz), NR1 (mouse mAb clone 54.1; 1/1000; BD Biosciences), GluR1 (rabbit mAb clone C3T; 1/500; Millipore), GluR2/3 (rabbit poly; 1/1000; Chemicon), postsynaptic density (PSD)-95 (mouse mAb clone 7E3-1B8; 1:1000; Millipore), CaMKII (mouse mAb clone 6G9; 1/2000; Affinity Bioreagents), LIMK-1 (rabbit poly; 1/100; Santa Cruz), phospho-LIMK1 (Thr508)/LIMK2 (Thr505; rabbit poly; 1/500; Cell Signaling Technology) and Cofilin (rabbit poly; 1/500; Millipore).

Techniques: Western Blot